Effects of helicobacter pylori-HSP60-induced regulatory T cell on the progression of atherosclerotic plaque in mice

Objective To explore the induction of Helicobacter pylori-HSP60-induced CD4~+ CD25~+ T cell in vitro and its effect on the formation of atherosclerotic plaque. Methods Immature dendritic cells were extracted and induced from mouse bone marrow and then used to induce Helicobacter pylori-HSP60 CD4~+ CD25~+ T cells in vitro. After infusing the CD4~+ CIY25~+ T cells into homogenous mice, the size of atherosclerotic plaques was measured. Results Compared with control group, the expression of CDSO (43.0%) and CD86 (41.1%) on aspirin-treated dendritic cells was down-regulated. Immature dendritic cells induced more specific regulatory cells (13.0±1.94)% than the mature ones. These cells signiticantlty suppressed the proliferation. The size of atherosclerotic plaques (2.37±0.96) mm~2 in treated mice were smaller than untreated ones (P< 0.05). Conclusion Immature dendritic cells can be used to induce HSP60 specific regulatory T cells in vitro and the latter also can inhibit the progession of atherosclerosis. Key words: Helicobacter pylori (H. pylori); Heat shock protein60 (HSP60); Regulatory T cell; Atherosclerosis