2017•Zhonghua shiyan waike zazhiRequires access
Effect of microRNA-223 on proliferation and migration of pancreatic cancer cell lines PANC-1 and miaPaCa-2
Cheng Huang, Longhui Xiong, Yumin Xu, Ke Hu, Guangnian Ma, Xinbing Chen, Dawei Liu, De He
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Abstract
Objective
To identify the effects of microRNA (miRNA, miR)-223 on proliferation and migration of pancreatic cancer cells.
Methods
miR-223 mimic and inhibitor were transfected into PANC-1 and miaPaCa-2 cell lines. The expression level of miR-223 was determined by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR). Cell proliferation was evaluated by methyl thiazol tetrazolium (MTT) assay. Transwell migration assy was performed for cell migration ability.
Results
The expression of miR-223 in the PANC-1 and miaPaCa-2 cell lines was significantly higher than that in normal pancreatic cells (8.21±0.83, 11.22±0.72, tPANC-1=14.886, P=0.000; tmiaPaCa-2=24.025, P=0.000). FQ-PCR showed the miR-223 expression level in PANC-1 and miaPaCa-2 cell lines were increased significantly after transfection with miR-223 mimics (8.18±0.82, 10.51±0.79, tPANC-1=15.221, P=0.001; tmiaPaCa-2=20.765, P=0.001). The number of pancreatic cancer cells in miR-223 tranfected group was extremely increased as compared with control group (1.43±0.07 vs. 0.49±0.08, t=15.715, P=0.000; 1.55±0.07 vs. 0.63±0.05, t=19.283, P=0.000). The number of migrated PANC-1 and miaPaCa-2 cells in miR-223 tranfected group was significantly greater than that in miR-23 control group (164.20±8.45 vs. 73.50±4.50, t=16.419, P=0.000; 133.45±7.75 vs. 56.40±6.25, t=13.401, P=0.000). The proliferation of pancreatic cancer was significantly decreased in miR-223 inhibitor transfected group as compared with that in miR-23 control group (0.73±0.06 vs. 1.04±0.05, t=6.754, P=0.000; 0.77±0.04 vs. 1.13±0.08, t=7.321, P=0.000). Transell results showed less cells in miR-223 inhibitor transfected group than in control group (35.30±3.36 vs. 72.60±4.10, t=16.419, P=0.000; 32.50±2.75 vs. 57.40±3.35, t=9.953, P=0.000).
Conclusion
The overexpression of miR-223 could remarkably promote the proliferation and migration abilities of human pancreatic cancer PANC-1 and miaPaCa-2 cells.
Key words:
MicroRNA-223; Pancratic cancer; Proliferation; Migration