2006•Zhonghua fangshe yixue yu fanghu zazhiRequires access
Establishment of murine model of radiation-induced oral mucositis
Chongjin Feng, Junbing Guo, Li Chuyu, Hanyu Wang, Chunyang Li, Cao Xinping, Bin Cheng
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Abstract
ObjectiveTo establish a murine model of radiation-induced oral mucositis. Methods Left-sided buccal mucosa of 70 Sprague-Dawley rats were irradiated with X-rays (10 Gy/d), then six rats, selected randomly, were sacrificed at the 2nd, 4th, 6th, 8th, 14th, and 21st day after the irradiation, left-sided buccal mucosa were excised, corresponding irradiation dose for the selected rats were 20, 40, 60 and 80 Gy following radiation. The right buccal mucosa was excised and treated as its auto-controls tissue. Results Erythema was observed in the left-sided buccal mucosa in rates irradiated with X-rays of 60 Gy; a single or more ulcers observed in rates irradiated with 80 Gy X-rays; a large area of ulcer was observed at 4th day after 80 Gy irradiation. The radiation-induced ulcer in buccal mucosa was essentially recovered after about 2 weeks following 80 Gy irradiation. ConclusionThis murine model of radiation-induced oral mucositis was useful and practical in experimental studies.