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2012•Chin J Naut Med & Hyperbar MedRequires access

Effect of hyperbaric oxygen on angiogenesis in the brain of adult rats with cerebral ischemia-reperfusion injury

Qinghua Chen, Ya Li, Wenlan Wang, Jie Ren, Xiaoping Xie, Heng‐Gui Chen, Peng-fei Shi, Zhiwen Deng, Jinsheng Li

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Abstract

Objective To investigate the effect of hyperbaric oxygen (HBO) on angiogenesis in the brain of adult rats with cerebral ischemia-reperfusion injury.Methods The adult male Sprague-Dawley (SD)rats were randomly divided into 3 groups:the sham surgery (SS) group,the ischemia-reperfusion (IR) group and the HBO group,and each group was further divided into 3 subgroups:the 3- day group,the 7- day group and the 10-day group.Middle cerebral artery occlusion (MCAO) was performed on the IR and HBO groups to develop the animal model.One and half hours later,reperfusion was made.The HBO group received HBO treatment and the SS group was left there without any treatment.All the rats were sacrificed respectively on day 3,7,10 and 21.Cerebral samples were collected and treated with immunohistochemical staining for the monitoring of vascular endothelial growth factor (VEGF),VEGF receptor-1 (VEGFR-1) and CD34.The changes in the ischemia region were observed under the optical microscope,and pictures were taken.Results When compared with those of the SS group,counts of VEGF and VEGFR-1 and SD34 positive cells increased markedly in the IR group (P ＜ 0.05 ).Following HBO treatment,counts of VEGF,VEGFR-1 and SD34positive cells in the animals of the HBO group increased significantly on day 7 and 10,when compared with those of the IR group (P＜0.05).For the 10-day sub-group in the IR group,counts of VEGF were 17.3 ±2.6,those of VEGFR-1 18.5 ± 2.9 and those of CD34 11.3 ± 2.7 ; for the 10-day sub-group in the SS group,counts of VEGF were 4.2 ±0.8,those of VEGFR-1 4.2 ±0.3 and those of CD34 3.6 ±0.6; and for the 10-day sub-group in the HBO group,counts of VEGF were 43.6 ± 5.5,those of VEGFR-1 36.5 ± 5.8 and those of CD34 43.6 ±5.5.Conclusions HBO therapy could effectively promote angiogenesis of micro vessels in the brain of adult rats with ischemia-reperfusion injury. Key words: Hyperbaric oxygen;  Middle cerebral artery occlusion;  Angiogenesis;  VEGF; VEGFR-1

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Objective To investigate the effect of hyperbaric oxygen (HBO) on angiogenesis in the brain of adult rats with cerebral ischemia-reperfusion injury.Methods The adult male Sprague-Dawley (SD)rats were randomly divided into 3 groups:the sham surgery (SS) group,the ischemia-reperfusion (IR) group and the HBO group,and each group was further divided into 3 subgroups:the 3- day group,the 7- day group and the 10-day group.Middle cerebral artery occlusion (MCAO) was performed on the IR and HBO groups to develop the animal model.One and half hours later,reperfusion was made.The HBO group received HBO treatment and the SS group was left there without any treatment.All the rats were sacrificed respectively on day 3,7,10 and 21.Cerebral samples were collected and treated with immunohistochemical staining for the monitoring of vascular endothelial growth factor (VEGF),VEGF receptor-1 (VEGFR-1) and CD34.The changes in the ischemia region were observed under the optical microscope,and pictures were taken.Results When compared with those of the SS group,counts of VEGF and VEGFR-1 and SD34 positive cells increased markedly in the IR group (P ＜ 0.05 ).Following HBO treatment,counts of VEGF,VEGFR-1 and SD34positive cells in the animals of the HBO group increased significantly on day 7 and 10,when compared with those of the IR group (P＜0.05).For the 10-day sub-group in the IR group,counts of VEGF were 17.3 ±2.6,those of VEGFR-1 18.5 ± 2.9 and those of CD34 11.3 ± 2.7 ; for the 10-day sub-group in the SS group,counts of VEGF were 4.2 ±0.8,those of VEGFR-1 4.2 ±0.3 and those of CD34 3.6 ±0.6; and for the 10-day sub-group in the HBO group,counts of VEGF were 43.6 ± 5.5,those of VEGFR-1 36.5 ± 5.8 and those of CD34 43.6 ±5.5.Conclusions HBO therapy could effectively promote angiogenesis of micro vessels in the brain of adult rats with ischemia-reperfusion injury. Key words: Hyperbaric oxygen;  Middle cerebral artery occlusion;  Angiogenesis;  VEGF; VEGFR-1

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Available abstract

Objective To investigate the effect of hyperbaric oxygen (HBO) on angiogenesis in the brain of adult rats with cerebral ischemia-reperfusion injury.Methods The adult male Sprague-Dawley (SD)rats were randomly divided into 3 groups:the sham surgery (SS) group,the ischemia-reperfusion (IR) group and the HBO group,and each group was further divided into 3 subgroups:the 3- day group,the 7- day group and the 10-day group.Middle cerebral artery occlusion (MCAO) was performed on the IR and HBO groups to develop the animal model.One and half hours later,reperfusion was made.The HBO group received HBO treatment and the SS group was left there without any treatment.All the rats were sacrificed respectively on day 3,7,10 and 21.Cerebral samples were collected and treated with immunohistochemical staining for the monitoring of vascular endothelial growth factor (VEGF),VEGF receptor-1 (VEGFR-1) and CD34.The changes in the ischemia region were observed under the optical microscope,and pictures were taken.Results When compared with those of the SS group,counts of VEGF and VEGFR-1 and SD34 positive cells increased markedly in the IR group (P ＜ 0.05 ).Following HBO treatment,counts of VEGF,VEGFR-1 and SD34positive cells in the animals of the HBO group increased significantly on day 7 and 10,when compared with those of the IR group (P＜0.05).For the 10-day sub-group in the IR group,counts of VEGF were 17.3 ±2.6,those of VEGFR-1 18.5 ± 2.9 and those of CD34 11.3 ± 2.7 ; for the 10-day sub-group in the SS group,counts of VEGF were 4.2 ±0.8,those of VEGFR-1 4.2 ±0.3 and those of CD34 3.6 ±0.6; and for the 10-day sub-group in the HBO group,counts of VEGF were 43.6 ± 5.5,those of VEGFR-1 36.5 ± 5.8 and those of CD34 43.6 ±5.5.Conclusions HBO therapy could effectively promote angiogenesis of micro vessels in the brain of adult rats with ischemia-reperfusion injury. Key words: Hyperbaric oxygen;  Middle cerebral artery occlusion;  Angiogenesis;  VEGF; VEGFR-1

Key concepts: Ischemia, Medicine, Angiogenesis, Vascular endothelial growth factor, Reperfusion injury, Anesthesia, Hyperbaric oxygen, Immunohistochemistry

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Effect of hyperbaric oxygen on angiogenesis in the brain of adult rats with cerebral ischemia-reperfusion injury — Research Paper | ScholarLens