Parathyroid hormone on the adipogenic potential of rat bone marrow mesenchymal stem cells

Objective To observe the effect of different concentrations of parathyroid hormone 1-34 on the adipogenic potential of rat bone marrow mesenchymal stem cells (BMSCs). Methods ① Rat bone marrow mesenchymal cells were separated and expanded by adherent culture. The morphology of cells was observed and cell surface markers were examined by flow cytometry.② The multi-lineage differentiation capability of cells was examined by culturing cells under conditions favorable for adipogenic and osteogenic differentiation. ③ Taken P3 of BMSCs for test, different concentrations of PTH1-34 (0, 10-10, 10-9, 10-8 mol/L) were used to stimulate BMSCs respectively, 14 days later, lipoprotein lipase (LPL) activity were measured by enzyme linked immuno-sorbent assay (ELISA), mRNA expression of alkaline phosphatase (ALP) and PPARγ-2 were measured by realtime polymerase chain reaction (PCR).④Statistical analysis: data were presented as ±s. All statistical analysis was performed with windows Statistical Praduct and Serice Solutions (SPSS) 13.0. One-way analysis of variance (ANOVA) was applied to determine the difference between groups. Least signisicant difference (LSD) was used to determine the difference between the two randomized groups. Differences were considered significant at a value of P<0.05. Results The cells expressed CD44, CD29 but without expression of CD45. By culturing in adipogenic medium for 3 weeks and in osteogenic medium for 4 weeks respectively, and then identified by oil red O and Alizarin red, the cells were successfully induced to adipocytes and osteogenesis. Expressions of LPL were 11.20±0.16, 7.62±0.48, 5.84±0.57, 5.32±0.52, mRNA expressions of PPAR7-2 were 2.80±0.05, 1.36±0.23, 0.94±0.11, 0.78±0.04, ALP activity were 0.191 ±0.016, 0.333±0.024, 0.549±0.025, 0.684±0.021 respectively. Compared with the control group, different concentrations of PTH1-34 groups could decrease mRNA expression of LPL and PPARγ-2. ALP activity were increased(P<0.05). Conclusion PTH1-34 inhibits BMSCs of adipogenic differentiation and promotes osteogenic differentiation in a dose-dependent manner. Key words: Teriparatide; Mesenchymal stem cells; Bone marrow; Adipogenic differentiation; Osteogenic differentiation