Inhibition of PC cell-derived growth factor expression by shRNA transfection increases sensitivity of pancreatic cancer cell lines panc-1 to gemcitabine

Objective To detect the effects of PC cell-derived growth factor (PCDGF) on sensitivity of pancreatic cancer cell lines Panc-1 to gemcitabine by observing the changes after transfection. Methods The expression of PCDGF was detected in pancreatic cancer tissues and nonearcinoma tissues by using immunohistochemical staining and quantitative reverse transcription PCR ( t = 11.41, P < 0.05 ). Two sites of shRNA targeted to PCDGF were combined to pGenesil-1 vector,and transfected to Pane-1 with lipofectamine2000,and the sensitivity of cancer cell lines to gemcitabine was measured. Results The expression level of PCDGF in pancreatic cancer tissues was higher than in pancreatic cytoadenoma and necrosis tissues in pancreatitis. Transfection of shRNA targeted to PCDGF into Panc-1 cells could inhibit the PCDGF expression ( inhibition rate = 82.3% ). After transfection, the toxicity of gemcitabine to Panc-1 was remarkably increased especially in high concentration. Conclusion shRNA targeting PCDGF makes pancreatic cancer cell lines Panc-1 more sense to gemcitabine. Key words: PC-cell derived growth factor; Pancreatic carcinoma; Drug sensitivity; RNA interfere