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2014•Central Plains Medical JournalRequires access

Compound K induced apoptosis of gastric carcinoma cells and the effect on expression of apoptosis-related genes

Bo Li, Li Mao, Xia Dong, Rui Wang, Qingjun Lu

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Abstract

Objective To investigate the effect of CK on proliferation,apoptosis of BGC823 cells.Methods BGC823 cells were treated with CK at different concentrations,cell viability was assayed using MTT methods.Cell cycle,apoptosis rate were assayed by flow cytometry.The expression of apoptosis-related protein were detected by Western blotting.Results After BGC823 cells were treated with 5 μmol/L CK for 48 h,the growth cycle was arrested on G2/M phase,the apoptosis rate was (21.17 ± 3.16)％.The expressions of Bcl-2,Bid protein was down-regulated after CK treatment,while the Fas and Fas-L protein were not significantly changed.Conclusions CK inhibits proliferation of BGC823 cells,and induces apoptosis via mitochondrion-mediated pathway. Key words: Compound K;  Gastric carcinoma ;  Cell apoptosis

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What this paper is about

Objective To investigate the effect of CK on proliferation,apoptosis of BGC823 cells.Methods BGC823 cells were treated with CK at different concentrations,cell viability was assayed using MTT methods.Cell cycle,apoptosis rate were assayed by flow cytometry.The expression of apoptosis-related protein were detected by Western blotting.Results After BGC823 cells were treated with 5 μmol/L CK for 48 h,the growth cycle was arrested on G2/M phase,the apoptosis rate was (21.17 ± 3.16)％.The expressions of Bcl-2,Bid protein was down-regulated after CK treatment,while the Fas and Fas-L protein were not significantly changed.Conclusions CK inhibits proliferation of BGC823 cells,and induces apoptosis via mitochondrion-mediated pathway. Key words: Compound K;  Gastric carcinoma ;  Cell apoptosis

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Available abstract

Objective To investigate the effect of CK on proliferation,apoptosis of BGC823 cells.Methods BGC823 cells were treated with CK at different concentrations,cell viability was assayed using MTT methods.Cell cycle,apoptosis rate were assayed by flow cytometry.The expression of apoptosis-related protein were detected by Western blotting.Results After BGC823 cells were treated with 5 μmol/L CK for 48 h,the growth cycle was arrested on G2/M phase,the apoptosis rate was (21.17 ± 3.16)％.The expressions of Bcl-2,Bid protein was down-regulated after CK treatment,while the Fas and Fas-L protein were not significantly changed.Conclusions CK inhibits proliferation of BGC823 cells,and induces apoptosis via mitochondrion-mediated pathway. Key words: Compound K;  Gastric carcinoma ;  Cell apoptosis

Key concepts: Apoptosis, Flow cytometry, Cell cycle, Molecular biology, Blot, MTT assay, Cell, Cell growth

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Compound K induced apoptosis of gastric carcinoma cells and the effect on expression of apoptosis-related genes — Research Paper | ScholarLens