[miR-153-3p inhibits proliferation and migration of breast cancer cells via down-regulating ROCK1].

assay. Results Software-predicted results showed a continuous binding region between miR-153-3p and Rho-associated coiled-coil containing protein kinase 1 (ROCK1). The miR-153-3p mimics co-transfected with ROCK1 wild-type reporter vector significantly decreased the relative activity of luciferase in MDA-MB-231 cells. The transfection of hsa-miR-153-3p mimics increased miR-153-3p expression and decreased ROCK1 protein expression. In comparison with the control cells, the proliferation, migration and invasion of MDA-MB-231 cells over-expressing miR-153-3p were dramatically inhibited. Conclusion The miR-153-3p can inhibit the proliferation, migration and invasion of breast cancer cells via down-regulation of ROCK1.