2017•PubMedOpen access

[Repair effects of rat adipose-derived stem cells on DNA damage induced by ultraviolet in chondrocytes].

Jinli Zhang, Zhihe Liu, Wenbin Tang, Xifeng Xiong, Zhi Zhang, Wenjuan Cao, Xiaojian Li

Abstract

Objective: To explore the DNA repair effect of rat adipose-derived stem cells (ADSCs) on chond-rocytes exposed to ultraviolet (UV) radiation. Methods: UV and cultured with normal medium (irradiated group), and medium containing the ADSCs supernatant (ADSCs supernatant group) or ADSCs was used for co-culture (ADSCs group) for 24 hours; no irradiation chondrocytes served as control group. The cell proliferation was estimated by MTS method. The expression of phosphorylated histone family 2A variant (γH2AX) was detected by immunofluorescence and Western blot. Results: >0.05). Conclusion: ADSCs can increase the cell proliferation and down-regulate the γH2AX protein expression of irradiated cells, indicating ADSCs contribute to the repair of irradiated chondrocyte.

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Key concepts: Molecular biology, Stem cell, Adipose tissue, Collagenase, Chemistry, Staining, Chondrocyte, Flow cytometry