HPLC-UV method for simultaneous determination of sparfloxacin and dexamethasone sodium phosphate in eye drops.

A simple, sensitive liquid chromatographic method was developed and validated for the simultaneous estimation of sparfloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations. Optimum separation was achieved in less than 10 min using a C18 column (250 mmx4.6 mm i.d, 5μ particle size) by isocratic elution. The mobile phase consisting of a mixture of mixed phosphate buffer (pH 6.8) and acetonitrile (50:50, v/v) was used. Column effluents were monitored at 224nm at a flow rate of 1ml/min. Retention times of sparfloxacin and dexamethasone sodium phosphate were 3.01 and 6.47 min respectively. The linearity of sparfloxacin and dexamethasone sodium phosphate was in the range of 3-18μg/ml and 1-6μg/ml respectively. Developed method was economical because, the time taken and amount of solvent consumed for each analysis was less. The method was validated and was applied to the simultaneous determination of sparfloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations.