Toxicity of Bacillus thuringiensis Local Isolate Auky Island Padaido District in Biak Numfor Regency (ABNP 8, 9, 11 and 12) against Anopheles Mosquito Larvae by Deposition Method in the Field

Vector control of infectious diseases in biology continues to be encouraged to reduce the use of insecticides that have an impact on the environment. Biological Control using Bacillus thuringiensis bacteria local indigenous isolates continues to be developed, in this study the toxicity test of local isolates ABNP 8, 9, 11 and 12 on the larvae of Anopheles mosquito at the Faculty of Mathematics and Sciences, Cenderawasih University. The aim of the study was to determine the toxicity of 8, 9, 11, and 12 local ABNP isolates, and to obtain local indigenous isolates with toxicity ≥ 85% to Anopheles mosquito larvae. The method used was experimental with a completely randomized design pattern using 4 local isolates; the course of the study was made of 50 cm deep grooves, 40 cm in diameter, given transparent plastic and 10 liters of water with a height of 30 cm and then sprayed with 100 ml isolate culture. Each typical mosquito plastic containing 40 instar 2 Anopheles larvae, each treatment isolate was repeated 3 times, observations were made after 72 hours by counting the number of dead Anopheles larvae. The results showed the toxicity of local ABNP8 isolates (73.33%), 9 ABNP, (74.17%), 11 ABNP, (73.33) and 12 ABNP, (98.33%), and variance analysis showed that the isolate treatment differed significantly at the level of 05%. , further testing using BNt α.05, obtained by BNt Value 9.24. The results of variance analysis with BNt further tests showed the treatment of local isolates of B. thuringienis ABNP 8, 9, and 11 toxicity was not different from the death of Anopheles mosquito larvae, this could be caused by several factors, namely the possibility of ABNP 8,9 isolate, and 11 bacterial strains were the same by sex influential in metamorphosis. Whereas local B. thuringiensis ABNP 12 isolates with a toxicity of ≥ 85% are much higher than ABNP 8, 9 isolates and 11 may be caused by differences in toxicity and strains against the death of Anopheles larvae, considering B. thuringiensis based on current serological tests obtained 58 strains with toxicity different or specific to insect larvae.