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2018•PubMedOpen access

[Effect of miR-21 down-regulated by H 2O 2 on osteogenic differentiation of MC3T3-E1 cells].

Jianqiang Peng, Niansheng Huang, Sheng Huang, Liangping Li, Zemin Ling, Song Jin, Aijun Huang, Kun Lin, Xuenong Zou

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Abstract

Objective: on osteogenic differentiation of MC3T3-E1 cells. Methods: +osteogenic induction+siRNA-PTEN group (group D2). The osteogenesis related genes were detected by RT-PCR and the extracellular calcium deposition was detected by alizarin red staining. Results: <0.05). The extracellular calcium deposition in group D2 was obviously more than those in groups B2 and C2. Conclusion: inhibits the osteogenic differentiation of MC3T3-E1 cells, which may be induced by down-regulating the expression of miR-21.

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Objective: on osteogenic differentiation of MC3T3-E1 cells. Methods: +osteogenic induction+siRNA-PTEN group (group D2). The osteogenesis related genes were detected by RT-PCR and the extracellular calcium deposition was detected by alizarin red staining. Results: <0.05). The extracellular calcium deposition in group D2 was obviously more than those in groups B2 and C2. Conclusion: inhibits the osteogenic differentiation of MC3T3-E1 cells, which may be induced by down-regulating the expression of miR-21.

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Available abstract

Objective: on osteogenic differentiation of MC3T3-E1 cells. Methods: +osteogenic induction+siRNA-PTEN group (group D2). The osteogenesis related genes were detected by RT-PCR and the extracellular calcium deposition was detected by alizarin red staining. Results: <0.05). The extracellular calcium deposition in group D2 was obviously more than those in groups B2 and C2. Conclusion: inhibits the osteogenic differentiation of MC3T3-E1 cells, which may be induced by down-regulating the expression of miR-21.

Key concepts: Osteopontin, RUNX2, Chemistry, Tensin, Alkaline phosphatase, Molecular biology, Transfection, Western blot

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[Effect of miR-21 down-regulated by H 2O 2 on osteogenic differentiation of MC3T3-E1 cells]. — Research Paper | ScholarLens