2013Kenya VeterinarianRequires access

Evaluation of the sensitivity and specificity of latex agglutination and complement fixation tests in the field diagnosis of contagious bovine

MO Odongo, MM Mutuku, Martin Mwirigi, L Mwendwa

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Abstract

The sensitivity and specificity of latex agglutination test (LAT) and Complement fixation test (CFT) for the field diagnosis of Contagious Bovine Pleuropneumonia were determined using 90 bovine sera from various parts of the country. Of the ninety samples tested, 17 (18.9%) were positive in both tests, 2 (2.2%) were negative in both tests, whereas 65 (72.2%) were negative in CFT but positive in LAT and 6 (6.7%) were negative in LAT but positive in CFT. Overall, LAT was more sensitive than CFT as 67 (74.4%) of the samples were positive in LAT, whereas only 19 (21.1%) samples were positive in CFT. In contrast, CFT (25%) had a higher specificity than LAT (3%)

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What this paper is about

The sensitivity and specificity of latex agglutination test (LAT) and Complement fixation test (CFT) for the field diagnosis of Contagious Bovine Pleuropneumonia were determined using 90 bovine sera from various parts of the country. Of the ninety samples tested, 17 (18.9%) were positive in both tests, 2 (2.2%) were negative in both tests, whereas 65 (72.2%) were negative in CFT but positive in LAT and 6 (6.7%) were negative in LAT but positive in CFT. Overall, LAT was more sensitive than CFT as 67 (74.4%) of the samples were positive in LAT, whereas only 19 (21.1%) samples were positive in CFT. In contrast, CFT (25%) had a higher specificity than LAT (3%)

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Available abstract

The sensitivity and specificity of latex agglutination test (LAT) and Complement fixation test (CFT) for the field diagnosis of Contagious Bovine Pleuropneumonia were determined using 90 bovine sera from various parts of the country. Of the ninety samples tested, 17 (18.9%) were positive in both tests, 2 (2.2%) were negative in both tests, whereas 65 (72.2%) were negative in CFT but positive in LAT and 6 (6.7%) were negative in LAT but positive in CFT. Overall, LAT was more sensitive than CFT as 67 (74.4%) of the samples were positive in LAT, whereas only 19 (21.1%) samples were positive in CFT. In contrast, CFT (25%) had a higher specificity than LAT (3%)

Key concepts: Complement fixation test, Latex fixation test, Contagious bovine pleuropneumonia, Agglutination (biology), Haemophilus, Virology, Medicine, Biology

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Evaluation of the sensitivity and specificity of latex agglutination and complement fixation tests in the field diagnosis of contagious bovine — Research Paper | ScholarLens