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2020•IntechOpen eBooksOpen access

Agrobacterium-Mediated Transformation

Risha Amilia Pratiwi, Muhammad Imam Surya

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Abstract

Agrobacterium-mediated transformation (AMT) heavily relies on the capability of bacterial pathogen Agrobacterium tumefaciens in transferring foreign genes into a wide variety of host plants. Currently, AMT is the most commonly used method for generating transgenic plants. On the other hand, A. tumefaciens was very useful for plant breeding. It also accelerated the technology of plant breeding to obtain specific characters. Gene transfer from bacteria to plants is a complex mechanism that involves several functional steps. This chapter will give brief information related to AMT mechanism, including the history of crown gall disease, the natural pathogenesis of A. tumefaciens, and the general protocol of AMT.

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What this paper is about

Agrobacterium-mediated transformation (AMT) heavily relies on the capability of bacterial pathogen Agrobacterium tumefaciens in transferring foreign genes into a wide variety of host plants. Currently, AMT is the most commonly used method for generating transgenic plants. On the other hand, A. tumefaciens was very useful for plant breeding. It also accelerated the technology of plant breeding to obtain specific characters. Gene transfer from bacteria to plants is a complex mechanism that involves several functional steps. This chapter will give brief information related to AMT mechanism, including the history of crown gall disease, the natural pathogenesis of A. tumefaciens, and the general protocol of AMT.

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Available abstract

Agrobacterium-mediated transformation (AMT) heavily relies on the capability of bacterial pathogen Agrobacterium tumefaciens in transferring foreign genes into a wide variety of host plants. Currently, AMT is the most commonly used method for generating transgenic plants. On the other hand, A. tumefaciens was very useful for plant breeding. It also accelerated the technology of plant breeding to obtain specific characters. Gene transfer from bacteria to plants is a complex mechanism that involves several functional steps. This chapter will give brief information related to AMT mechanism, including the history of crown gall disease, the natural pathogenesis of A. tumefaciens, and the general protocol of AMT.

Key concepts: Agrobacterium tumefaciens, Gall, Agrobacterium, Transformation (genetics), Biology, Gene transfer, Host (biology), Genetically modified crops

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