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2017•Journal of Stem Cells and Regenerative MedicineOpen access

Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton's Jelly

Peng Yew Kenny Boey, Say Liang Daniel Lim, Kin Fai Tang, Ming Ming Li, Andrew K. Ekaputra, Prosanto Kumar Chowdhury, Rajat Anand Gopal Mukherjee, Jennifer Teo, Arvin C Faundo, Yoke Fong Chiew

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Abstract

The Wharton's Jelly (WJ) is an established source of mesenchymal stem cells (MSC).We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods.The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers.Consequently, we investigated if mechanical or enzymatic dissociation of MSC from cryopreserved WJ is superior over the explant method.

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The Wharton's Jelly (WJ) is an established source of mesenchymal stem cells (MSC).We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods.The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers.Consequently, we investigated if mechanical or enzymatic dissociation of MSC from cryopreserved WJ is superior over the explant method.

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Available abstract

The Wharton's Jelly (WJ) is an established source of mesenchymal stem cells (MSC).We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods.The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers.Consequently, we investigated if mechanical or enzymatic dissociation of MSC from cryopreserved WJ is superior over the explant method.

Key concepts: Wharton's jelly, Mesenchymal stem cell, CD90, Cryopreservation, Stem cell, Haematopoiesis, Biology, Andrology

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Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton's Jelly — Research Paper | ScholarLens