STUDY OF THE INFLUENCE OF SOLVENTS ON THE POLYPHENOLS CONTENT OF SALVIA OFFICINALIS L EXTRACTS AND THE EFFECT ON ANTIOXIDANT ACTIVITY
Soukaina Tidjani, Chaima Adaika, Selma Djaber
Abstract
Soukaina Tidjani, Chaima Adaika, Selma Djaber
Abstract
Algerian Salvia Officinalis L aerial parts were extracted with pure solvents in increasing polarity (hexane, acetone, methanol and 80% aqueous methanol. All extracts were examined by assessing their content in polyphenols and the antioxidant levels were evaluated by two in vitro tests using spectroscopic and the voltametric methods. The quantitative assessment of total phenols and flavonoids by the colorimetric method make it clear that the extracts are very rich of these compounds. The hydro-methanol and methanol extracts gave the highest DPPH and radicals scavenging activities (12.15 μg/ml) and (0.704 mg/ml) respectively.
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Algerian Salvia Officinalis L aerial parts were extracted with pure solvents in increasing polarity (hexane, acetone, methanol and 80% aqueous methanol. All extracts were examined by assessing their content in polyphenols and the antioxidant levels were evaluated by two in vitro tests using spectroscopic and the voltametric methods. The quantitative assessment of total phenols and flavonoids by the colorimetric method make it clear that the extracts are very rich of these compounds. The hydro-methanol and methanol extracts gave the highest DPPH and radicals scavenging activities (12.15 μg/ml) and (0.704 mg/ml) respectively.
Key concepts: Methanol, Chemistry, Polyphenol, Acetone, DPPH, Phenols, Antioxidant, Chromatography