2018•PubMedRequires access

[Effects of Different Promoters and MAR Combinations on Transgene Expression of Recombinant CHO Cells].

Qin Li, Xiao-Yin Wang, Chun‐Peng Zhao, Zheng‐Wei Tian, Danhua Xu, Tianyun Wang, Junhe Zhang

Abstract

OBJECTIVE: To analyze the effects of different promoters and matrix attachment region (MAR) on the expression of transgene in Chinese hamster ovary (CHO) cells. METHODS: were analyzed by qPCR. RESULTS: on one side and both sides were 3.68-fold and 9.25-fold,respectively. CONCLUSION: can enhance the expression levels of transgene,which may be related to the increase of gene copy number.

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Key concepts: Molecular biology, Chinese hamster ovary cell, Promoter, Green fluorescent protein, Transgene, Flow cytometry, Transfection, Biology