Fetal bovine serum의 농도에 따른 infectious hematopoietic necrosis virus와 infectious pancreatic necrosis virus의 감염가 변화

Fetal bovine serum (FBS) is an essential element of cell growth and can also affect the viral replication. In this study, we tried to find out whether FBS concentration affects the viral infectivity titer of IHNV and IPNV. EPC cells were suspended with MEM supplemented with various concentrations of FBS (MEM0, MEM2, MEM5 and MEM10) and cultured in 96-well plate. Each virus was 10-fold diluted virus and inoculated in 96-well plate. The highest infectivity titer of IHNV was 107.88 TCID50/mL in 96-well plate using MEM5 and the lowest one was 107.30 TCID50/mL in 96-well plate using MEM10. The highest infectivity titer of IPNV was 107.47 TCID50/mL in 96-well plate using MEM5 and the lowest one was 106.97 TCID50/mL in 96-well plate using MEM10. This study showed that not only 0% FBS but 10% FBS leads low infectivity titer of IHNV and IPNV. Therefore, it is considered that the desirable concentration of FBS is 2% or 5% for measurement of infectivity titer of IHNV and IPNV.