The effect of fungicides on growth of Fusarium fungi in vitro

Four different fungicides that recommended to control seed-borne pathogens, were tested in this study. Three fungicides contain singly pyraclostrobin (200 g/L), thiram (400 g/L), fludioxonil (25 g/L), and one fungicide contains together imazalil, metalaxyl, and tebuconazole (50, 40, and 30 g/L, respectively). Comparative assessment of the fungicides at four concentrations of active substance (10, 100, 1 000, and 10 000 ppb) on the growth of ten strains of Fusarium spp. was studied in vitro tests. To visualize the effect of fungicides on fungal growth the strains were cultivated in 50 mL liquid Czapek medium containing fungicides in the range of concentrations. The fungicide based on pyraclostrobin was the most effective in growth inhibition of Fusarium fungi. Broadly, the using of this fungicide is confident way to control Fusarium species, which are serious threat to crop production. The sensitivity of F. acuminatum, F. graminearum, F. semitectum, F. culmorum, F. sporotrichioides, F. equiseti strains to fungicides was higher in compare with the strains belonging to F. oxysporum, F. solani, F. verticillioides, and F. proliferatum.