Protease Activity and Gel-forming Ability of Ginger Rhizome Juice
Norihiro Yamada, Yasushi Kokean, Kaori Umetani, Jun Shimizu, Osamu Kurita, Kazunobu Tsumura
Abstract
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Norihiro Yamada, Yasushi Kokean, Kaori Umetani, Jun Shimizu, Osamu Kurita, Kazunobu Tsumura
Abstract
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The ginger rhizome has been known to contain proteolytic enzymes. In this study, we evaluated the protease activities in juice extracted from ginger rhizomes. The specific protease activity in juice from ginger ripened after harvesting was higher than that of ginger that was not ripened after harvesting. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that the ginger rhizome juice displaying high specific protease activity contained a 30 kDa protein. This juice was also observed to have milk gel-forming ability.
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The ginger rhizome has been known to contain proteolytic enzymes. In this study, we evaluated the protease activities in juice extracted from ginger rhizomes. The specific protease activity in juice from ginger ripened after harvesting was higher than that of ginger that was not ripened after harvesting. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that the ginger rhizome juice displaying high specific protease activity contained a 30 kDa protein. This juice was also observed to have milk gel-forming ability.
Key concepts: Rhizome, Protease, Chemistry, Food science, Polyacrylamide gel electrophoresis, Enzyme, Fruit juice, Neutral protease