Sivas Yöresinde Sığır ve Kenelerde Theileria, Babesia ve Anaplasma Enfeksiyonlarının Araştırılması

This study was carried out to investigate the presence and distribution of Theileria, Babesia and Anaplasma species in cattle and their ticks from Sivas region. A total of 314 EDTA-blood samples and 610 ticks were analysed. A part of 18S and 16SrRNA genes of Theileria/Babesia and Anaplasma species were amplified from the genomic DNAs extracted of the blood samples and tick pools by with polymerase chain reaction (PCR). A total of 14 probes (two catchall, two genera and ten species-specific) were bound on a membrane and then the PCR products were tested by reverse line blot (RLB) assay. The partial sequences of the 18S and 16S rRNA genes of representative positive samples were determined. According to the results of the blood and tick samples analysed by RLB and sequencing, T. buffeli (GenBank accesion number: KJ183080), A. centrale (KJ183082), A. marginale (KJ183083), A. bovis (KJ183084), one Babesia genotype (Babesia sp. Sivas, KJ183081) and one Anaplasma genotype (Anaplasma sp. Sivas, KJ210855) were detected. Babesia sp. Sivas were found to be 99% identical with B. occultans, Babesia sp. Kashi and Babesia sp. Kayseri whereas Anaplasma sp. Sivas were found to be 99% and 98% identical with Anaplasma sp. Clone 7 and A. bovis, respectively. Overall prevalences of Theileria and Anaplasma infections in cattle were found to be 5.10% and11.15% by RLB, respectively. This study is the first molecular survey on species of Theileria, Babesia and Anaplasma in cattle and ticks from Sivas.