Comparative Characteristics of the Culture-enzymatic and Molecular-genetic Methods for Indicating Mollicutes – Mycoplasma hominis and Ureaplasma spp., Isolated in Patients with Acute Uncomplicated Pyelonephritis

The objective: is to analyze the frequency of detection of M.hominis and Ureaplasma spp. in the biological material of patients with acute uncomplicated pyelonephritis (AUP) by cultural-enzymatic and molecular-genetic methods and to prove their involvement in the development of the inflammatory process of the urinary and genital tracts.Materials and methods. The study involved 224 women of reproductive age, patients with AUP and concomitant chronic diseases of the pelvic organs. We examined urine samples, scrapings of the urethral mucosa and cervical canal using cultural-enzymatic (CEM) and polymerase chain reaction (PCR). Іn the serum of 217 patients determined the level of specific IgG-antibodies to mollicutes by enzymelinked immunosorbent assay.Results. The introduction of the PCR method for the detection of mollicutes in the urinary and genital tracts in patients with AUP revealed possible to increase the efficiency of their indication, primarily when M.hominis is detected. The frequency of Ureaplasma spp. detection by both methods was significantly higher than M.hominis in samples of biological material of different localization in the examined patients. A high frequency of coincidence in the detection frequency of M.hominis and Ureaplasma spp. was noted using two methods (PCR and CЕM): in the urine – 85.9% and 87.5%, in scrapings of the mucous membrane of the urethra – 81.3% and 93.3% and the cervical canal – 78.0% and 88.8%. The coincidence of diagnostic levels of antibodies to Ureaplasma spp. in the presence of this pathogen in the biological material studied in 65 (76.5%) of 85 patients were registered, and M.hominis – in 34 (41.0%) of 83 patients.Conclusion. The complex use of various methods of laboratory diagnostics allows obtaining accurate data on the presence of mycoplasma infection in patients with AUP and concomitant chronic diseases of the pelvic organs. The presence of diagnostically significant titers of IgG-antibodies to M.hominis and Ureaplasma spp. proves their influence on the course of the inflammatory process of the urinary and genital tracts of women with AUP.