Ptimization of Co-immobilization of Cellulase and β-glucosidase

Abstract Cellulase can hydrolyze cellulose to produce reducing sugars such as cellobiose and cellotriose. β-glucosidas can further hydrolyze cellobiose and cellobiose produced by cellulase to produce glucose. According to the hydrolysis mechanisms of cellulase and β-glucosidas, we selected two enzyems, cellulase and β-glucosidase to study optimization of co-immobilization of cellulase and β-glucosidas. Meanwhile, we selected two materials, activated carbon and sodium alginate to co-immobilize cellulase and β-glucosidas by the immobilization method of embedding-adsorption. The immobilization conditions, alginate, CaCl2 and activated carbon was optimized. The results showed that the optimum concentrations of sodium alginate and CaCl2 were 2% and 2%, respectively, and the quality of activated carbon is 0.15 g. The optimal ratio of cellulase to β - glucosidase was 1:1.5. The results indicated that cellulase and β-glucosidase had a synergistic effect and that their compound degradation of cellulose was better than the separate effects of the two enzymes acting independently.