1993•Journal of Computer-Assisted MicroscopyRequires access
Freeze-substitution for the preservation of cell-to-cell contact regions in the multicellular Dictyostelium discoideum slug
Manuela Fuchs, Malcolm K. Jones, Keith L. Williams
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Abstract
We use rapid freezing and freeze-substitution for the preservation of cell-to-cell contact regions in the multicellular Dictyostelium discoideum slug. The aim of this study is to obtain data on the ultrastructure of cells in the periphery of the slime mold and on the nature of cell junctions between the internal cells. The study revealed that the internal cells have close cell contacts. The plasma membranes have a characteristic trilaminar appearance.