In vitro Propagation of Indian Teak (Tectona grandis L.) from leaf explants

Efficient plant regeneration via direct organogenesis was established using leaf explants of Tectona grandis L. were tested for growth and organogenic capacity on MS medium fortified with various concentrations of 6-Benzyl Amino Purine and Kinetin (0.5–3.0mg/L) either singly or in various combinations of IAA/IBA (0.5–3.0 mg/L). The highest shoot regeneration frequency (100%) was obtained from leaf explants on MS medium fortified with (1.5 mg/L) IBA/IAA in combination of (2.0mg/L) BAP. At the end of 6 weeks the regenerated shoots were transferred on the same medium (MS + 1.5 mg/L IAA/IBA + 2.0 mg/L BAP) for further proliferation and elongation. The regenerated shoots were rooted with high frequency (60%) in MS medium supplemented with (1.5 mg/L) IBA when compared to other auxin IAA. The in vitro raised plantlets were successfully established in green house and transplanted to natural conditions with 70% survival.