Optimization of the extraction technology for flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.
Yanfang Wang, Haiyan Zhao, MA Yong-ping, Yuan FeiJuan
Abstract
Yanfang Wang, Haiyan Zhao, MA Yong-ping, Yuan FeiJuan
Abstract
[Objective] The aim was to study on the effects of different experimental conditions on the extraction rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE. [Method] Effects of ethanol content, solid-liquid ratio, and extraction time on the extraction rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE were studied by orthogonal test. Under the optimal extraction conditions, effects of ultrasonic power and ultrasonic time on the extraction rate of flavonoids were investigated by single factor test. [Result] Results of orthogonal test showed that the optimal extraction conditions for the flavonoids from RADIX RHIZOMA GLYCYRRHIZAE were 75% ethanol as solvent, 1∶14 solid-liquid ratio, and 2 h extraction time. Under these conditions, ultrasonic pretreatment of raw materials was conducted. And the optimal ultrasonic conditions were 100 W ultrasonic power and 1 h ultrasonic time. [Conclusion] Under the optimal extraction conditions, the extraction rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE reached 2.69%.
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[Objective] The aim was to study on the effects of different experimental conditions on the extraction rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE. [Method] Effects of ethanol content, solid-liquid ratio, and extraction time on the extraction rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE were studied by orthogonal test. Under the optimal extraction conditions, effects of ultrasonic power and ultrasonic time on the extraction rate of flavonoids were investigated by single factor test. [Result] Results of orthogonal test showed that the optimal extraction conditions for the flavonoids from RADIX RHIZOMA GLYCYRRHIZAE were 75% ethanol as solvent, 1∶14 solid-liquid ratio, and 2 h extraction time. Under these conditions, ultrasonic pretreatment of raw materials was conducted. And the optimal ultrasonic conditions were 100 W ultrasonic power and 1 h ultrasonic time. [Conclusion] Under the optimal extraction conditions, the extraction rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE reached 2.69%.
Key concepts: Radix (gastropod), Extraction (chemistry), Chromatography, Chemistry, Ultrasonic sensor, Raw material, Botany, Organic chemistry