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2019•International Journal of Current Microbiology and Applied SciencesOpen access

Molecular Characterization of Staphylococcus aureus Isolated from Foods of Animal Origin by Targeting Virulence and Antibiotic Resistance Genes

Devender Choudhary, Surendra Singh Shekhawat, Abhishek Gaurav, Hitesh Kumar, Nirmal Kumar

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Abstract

Meat, milk and egg are a major component in the human diet and milk is an important food for vegetarian class, but it also serves as a very good medium for the growth of many microorganisms including pathogenic bacteria (Ruegg, 2003). In the present study, samples of milk, egg and meat were collected from different areas of Udaipur city.PCR assay was standardized for the detection of species specific genes16SrRNA gene, virulence gene (tsst gene) and antibiotic resistance gene (ermC, tetK, and aacA-aphD) of S. aureus. The prevalence of S. aureus was found to be 37.5%, 5%, and 15% in milk, egg and meat respectively. The prevalence of 16SrRNA, tsst, ermC, tetK, and aacA-aphD genes were recorded as 100%, 21.73%, 13%, 26% and 21.7% respectively.

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Meat, milk and egg are a major component in the human diet and milk is an important food for vegetarian class, but it also serves as a very good medium for the growth of many microorganisms including pathogenic bacteria (Ruegg, 2003). In the present study, samples of milk, egg and meat were collected from different areas of Udaipur city.PCR assay was standardized for the detection of species specific genes16SrRNA gene, virulence gene (tsst gene) and antibiotic resistance gene (ermC, tetK, and aacA-aphD) of S. aureus. The prevalence of S. aureus was found to be 37.5%, 5%, and 15% in milk, egg and meat respectively. The prevalence of 16SrRNA, tsst, ermC, tetK, and aacA-aphD genes were recorded as 100%, 21.73%, 13%, 26% and 21.7% respectively.

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Available abstract

Meat, milk and egg are a major component in the human diet and milk is an important food for vegetarian class, but it also serves as a very good medium for the growth of many microorganisms including pathogenic bacteria (Ruegg, 2003). In the present study, samples of milk, egg and meat were collected from different areas of Udaipur city.PCR assay was standardized for the detection of species specific genes16SrRNA gene, virulence gene (tsst gene) and antibiotic resistance gene (ermC, tetK, and aacA-aphD) of S. aureus. The prevalence of S. aureus was found to be 37.5%, 5%, and 15% in milk, egg and meat respectively. The prevalence of 16SrRNA, tsst, ermC, tetK, and aacA-aphD genes were recorded as 100%, 21.73%, 13%, 26% and 21.7% respectively.

Key concepts: Staphylococcus aureus, Virulence, Microbiology, Antibiotics, Biology, Antibiotic resistance, Gene, Bacteria

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