Isolation of bacteria from Tuz Gölü lake that can grow on high salt concentration

Today, extremophile is widely studied by the scientist due to its strong survival features that allow them to survive under extreme environment. Halophile is one example that inhabit high salt concentration environment. Isolation of bacteria from the area of Tuz Gölü lake, also known as hypersaline lake in the central plateau of Turkey, led to the isolation of 4 halotolerant bacteria, which were able to grow optimally in media with 0–10% of salt. Surprisingly, the strain A-4 isolate was successfully isolated from the Tuz Gölü lake water on the minimal media that consists of 2,2-dichloropropionic acid as a carbon source. This indicated that the strain A-4 was very useful in the environmental remediation due to its capability to break down 2,2-DCP, a halocarboxylic found in herbicide. Further analysis such as biochemical tests and 16srRNA sequence analysis were necessary to further identify the species of the bacteria in the next experiment.