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2019•Chemical CommunicationsRequires access

DNAzyme-powered nucleic acid release from solid supports

Ting Cao, Yongcheng Wang, Ye Tao, Lexiang Zhang, Ying‐Lin Zhou, Xin‐Xiang Zhang, John A. Heyman, David A. Weitz

Open publisher page 3 citations

Abstract

-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

About this research paper

What this paper is about

-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

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OpenAlex reports 3 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

Key concepts: Deoxyribozyme, Cleave, Nucleic acid, Oligonucleotide, Chemistry, DNA, Cleavage (geology), Biochemistry

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