Analysis of the correlation between the quantities of hepatitis B virus covalently closed circular DNA and condition in chronic hepatitis B patients.

Objective To investigate the effect of HBV cccDNA quantitation on the condition of chronic hepatitis B(CHB) patients.Methods The quantity of HBV cccDNA and HBV DNA in the hepatocytes and HBV DNA in the serum of 42 patients with CHB were detected by real-time fluorescence quantitative PCR(FQ-PCR).The inflammation and fibrosis degree were estimated by pathological staining.The expression of HBsAg,HBcAg in the hepatocytes were identified by SP method.Correlations between the HBV cccDNA quantitation and other indexes were analyzed.Results The HBV cccDNA quantitation in the liver tissue were positively correlated to the HBV DNA levels in the liver tissue and sera(r=0.807,P0.001.r=0.627,P0.001),and were not correlated to the degree of liver inflammation and fibrosis.The HBV cccDNA quantitation in the liver tissue were not correlated to the expression of HBsAg,but positively correlated to the expresson of HBcAg(r=0.486,P0.05).Conclusions The quantitation of HBV cccDNA can reflect the replication of HBV more accurately,and plays important role in the diagnosis,antiviral treatment and withdrawal time selection of CHB.It is more reliable to take the quantification of hepatic tissue HBV cccDNA combining the HBV DNA in the serum and the expression degree of HBcAg as antivirus therapeutic indexes.