Screening of Xylanase-producing Strain

Objective To screen a strain with high production of xylanase from soil and study the prime xylanase-producing condition.Methods To screen xylanase high-producing strain,based on the size of hydrolytic ring on the selective medium and xylanase activity during shake flask fermentation.Through a series of single-factor experiments,the effects of carbon source,nitrogen source and inorganic salt on xylanase production were studied.Results A strain with high production of xylanase was obtained.The optimal carbon resource,nitrogen resource and inorganic salt for xylanase production were wheat bran(30 g/L),beef extract(10 g/L),K2HPO4(1.0 g/L).Conclusion This strain has a potential for the production of xylanase.