Optimization of Ultrasonic-Microwave Assisted Extraction of Chlorogenic Acid from Flos Lonicerae

The current study was carried out to optimize the ultrasonic-microwave assisted extraction of chlorogenic acid from Flos Lonicerae.The extraction yield of chlorogenic acid was investigated with respect to temperature,ethanol concentration,solid-to-solvent ratio,microwave power and irradiation time.The combined use of ultrasonic and microwave provided higher extraction efficiency than their individuals.An extraction yield of chlorogenic acid of 5.93% was obtained when ultrasonication for 30 min followed by microwave irradiation at a power of 400 W for 5 min was used for the extraction of Flos Lonicerae with 70% ethanol at a solid-to-solvent ratio of 1:15(g/mL).