2009•Journal of Guangxi Normal UniversityRequires access
CAT1,EAAC1 and PepT1 mRNA in Small Intestine in Different Parts of in Specific Distribution in Tibetan
Shi ChangYou, Shi-Yu Bin, Chu WuYing, Tang WanLin, Dunxue Chen
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Abstract
Six healthy Tibetan piglets of at 28 day,with similar weight were chosen to slaughter.Small intestinal segments samples were collected from duodenum,proximal and distal jejunums,ileum for detection intestinal tissue distribution of oligopeptide transporter PepT1,anionic amino acid transporter EAAC1 and cationic amino acid transporter CAT1 mRNA abundance by Real-time PCR.The results show the abudance of PepT1 mRNA in distal jejunum was significantly higher than in duodenum,proximal jejunum and ileum and the Ileum had the lower expression abundance.There is an increased expression gradient of EAAC1 mRNA from proximal to distal intestine.It's significantly higher in ileum than in duodenum,proximal and distal jejunums.Intestinal tissue distribution of CAT1 mRNA is similar to EAAC1.Expression abudance of CAT1 mRNA in ileum is the highest in intestinal segment.