Screening of Laccase-Producing Fungal Strain and the Preliminary Study on the Solid-State Fermentation Conditions

Objective To Screen the laccase-producing fungi and optimize its solid-state fermentation medium as well as study the property of crude enzyme.Methods The strains were obtained by plate screening and the optimization of medium was performed by measuring the activity of laccase.Results A fungal strain with a high laccase-producing activity was obtained under solid-state fermentation.The optimal medium contained:soybean meal and sawdust with the ratio of 1∶1,0.5 % xylose,2.0 % ammonium tartrate and 65 % moisture.The optimal temperature was 60 ℃ for laccase,and the laccase was stable at less than 50 ℃.The optimal pH value was 3.5,and the laccase was stable at pH 6.5～9.0.Conclusion A fungal strain with a high laccase-producing activity was obtained and the activity of laccase was increased 7 times with the optimization of medium.