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2019•PubMedOpen access

[Isolation of cancer stem cells and the establishment of a H 2O 2-resistant cancer stem cell model].

Qingxi Liu, Hongran Chen, Hui Li, Tong‐Cun Zhang, Wenjian Ma

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Abstract

OBJECTIVE: To isolate cancer stem cells (CST) from human breast cancer cell line (MCF-7) and study their sensitivity toward oxidative stress. METHODS: for 120 minutes. The expression of DNA damage marker histone H2AX phosphorylation (γH2AX) was detected by immunocytochemical staining. RESULTS: injury. CONCLUSION: Serum-free suspension culture medium can produce globular CST cells from MCF-7 tumor cell line, which have strong antioxidant damage.

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OBJECTIVE: To isolate cancer stem cells (CST) from human breast cancer cell line (MCF-7) and study their sensitivity toward oxidative stress. METHODS: for 120 minutes. The expression of DNA damage marker histone H2AX phosphorylation (γH2AX) was detected by immunocytochemical staining. RESULTS: injury. CONCLUSION: Serum-free suspension culture medium can produce globular CST cells from MCF-7 tumor cell line, which have strong antioxidant damage.

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Available abstract

OBJECTIVE: To isolate cancer stem cells (CST) from human breast cancer cell line (MCF-7) and study their sensitivity toward oxidative stress. METHODS: for 120 minutes. The expression of DNA damage marker histone H2AX phosphorylation (γH2AX) was detected by immunocytochemical staining. RESULTS: injury. CONCLUSION: Serum-free suspension culture medium can produce globular CST cells from MCF-7 tumor cell line, which have strong antioxidant damage.

Key concepts: Flow cytometry, Molecular biology, Cell culture, Cancer cell, Stem cell, Biology, Cancer stem cell, Staining

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[Isolation of cancer stem cells and the establishment of a H 2O 2-resistant cancer stem cell model]. — Research Paper | ScholarLens