Protective effect of Phytolacca acinosa polysaccharides I (PAP-I) on hematopoiesis in cyclophosphamide-treated and ~(6))Co-irradiated mice

Average survival time of mice treated with cyclophosphamide (CY)300mg/kg once alone was 13.3±7.2d(n=7), however, averge survival time of mice treated with CY 300 mg/kg in combination with PAP-1,10mg/kg, 3time/wk was 36.7±16.4d(n=7,P<0.01). PAP-1, ip had beneficial effect on the recovery of the CY induced decrease of peripheral leukocyte number, and the nucleated bone marrow cell (BMC) number and [~(3)H]TdR uptaken by BMC induced by rmGM-CSF in S180 bearing mice treated with CY, In mice, after the first ip treatment with CY 100mg/kg on d 7, the peripheral leukocyte number decreased on d9 and recovered to normal level about d13 to d15. Such recovery was accelerated by administrating PAP-1, 10mg/kg, 3 times/wk. A significant increase of the activity to form colony in spleen(colony-forming unit in spleen, CFU-S_(8), CUF-S_(12))in mice irradiated with 550 rad ~(Co) gamma-rays and an enhancement of proliferative response of BMC to rmGM-CSF treated with PAP-1, 10mg/kg, 3 times/wk, ip were observed. After PAP-1, 10mg/kg, ip once, a significant increase in the number of peripheral blood leukocytes and a rise in the serum of colony stimulating factor (CSF) were also confirmed. The types of CSF in serum were M-CSF and other hematopoietic growth factors, which were confirmed by using McAb of IL-3, GM-CSF and PcAb of M-CSF.