High affinity anti-TNF#alapha# human monoclonal antibodies directly selected from pooled phage display library

Human anti-TNF#alapha# monoclonal antibodies were directly selected from the pooled human antibody combinatorial libraries using phage display technology. Thre different assays proved that phage antibodies that could bind TNF#alapha# were enriched effectively after five rounds of biopanning. Preliminary identification of obtained phage clones indicated that 7 phage clone could bind human hTNF#alapha# effectively and specifically, and that Fabs derived from them also could bind with hTNF#alapha# effectively. Hence, we obtained human anti-TNF#alapha# monoclonal antibodies directly through phage display technology, laying a good foundation for further obtaining human anti-TNF#alapha# monoclonal antibodies which could be clinical employed.