2018•PubMedOpen access

[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].

Yan Xu, Bo Wei, Jin Zhou, Qingqiang Yao, Liming Wang, Jian Na

Abstract

Objective: chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs). Methods: were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds). The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR. Results: <0.05). Conclusion: can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.

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Key concepts: Polycaprolactone, Chondrogenesis, Chemistry, Mesenchymal stem cell, Aggrecan, Scaffold, Biomedical engineering, Cartilage