ISSR-Based Molecular Characterization of Opium poppy (Papaver somniferum L.) Genotypes

Opium poppy (Papaver somniferum L.) is an important medicinal crop in the world. In the present investigation 24 prominent opium accessions were assessed by ISSR molecular markers for the genetic diversity analysis. A total of 116 amplified bands were obtained from the 15 primers, out of which 98 were polymorphic. Overall size of PCR amplified products had ranged between 300 bp to 2800 bp. The polymorphism percentage ranged from 16.66% to 100% for nine primers. The ISSR similarity matrix revealed the Jaccard’s similarity values lie between 0.35-0.90. The 24 genotypes could be divided into two major clusters at a similarity coefficient of 0.47, Chetak Aphim sole genotype made single cluster while Cluster II included 23 genotypes. UPGMA method used for dendrogram exhibited the proper genetic diversity in all the 24 accessions of opium poppy. The present investigation revealed that significant diversity existed in all the genotypes of Opium poppy.