Production of Gladiolus grandiflorus by tissue culture technique

We achieved an economical conclusion for Laboratory propagation of gladiolus bulbs Gladiolus grandiflorus using tissue culture techniques. This method include the use of corm slices with 8-10 millimeters thickness which were lied in contact with Murashige and Skoog solid medium supported with 0.25, 0.5, 1.0 and 2.0 mg/L of NAA or 2,4-D interaction with different concentrations (0.5, 1.0, 2.0 and 4.0 mg/L) of BA and Kinetin. The number of shoots obtained at the level of 4.0 mg/L BA combined with 1.0 mg/L of NAA was 5.1 folds while the largest number of shoots 4.9 folds were found with the kin at 4.0 mg/L with 1.0 mg/L of NAA. The later has more impact on vegetative growth and the number of shoots formed than 2,4-D. The sucrose and NAA had a great effect on the rooting of shoots and formation of corms, through the best periods of time for each of them. Rooting were 100% when used 60 g/L sucrose within 14 days, and took 56 days to produce corms. When 90 g/L of sucrose were used with 10 mg/L NAA for rooting, shoots were rooted within 11 days and corm formation 7.0 corm/shoot were accomplished after 42 days. The corms were ready for planting in the filed.