ANTIBACTERIAL ACTIVITY OF KEMBANG BULAN (Tithonia diversifolia) LEAF EXTRACT ON Staphylococcus aureus

The aims of this study was to know about the antibacterial activity of Kembang Bulan (Tithonia diversifolia) leaf extract against Staphylococcus aureus. This study used eight isolated of S. aureus bacteria isolated from three dairy farms Surabaya. Samples of this study used Tithonia diversifolia leaf extract with concentration 10%, 5%, 2.5%, 1.25% and 0%. This study used disk diffusion method, and inhibition zone measured in millimeters to determine the antibacterial activty of Tithonia diversifolia leaf extract. Based on the result of this study, showed that eight samples (100%) are positive has antibacterial activity against S.aureus with 5% and 10% concentration of Tithonia diversifolia leaf extract due to the presence of sesquiterpenes in Tithonia diversifolia that has antibacterial activity. Meanwhile Tithonia diversifolia leaf extract with 0%, 1.25% and 2.5% did not showing any antibacterial activity against S.aureus.