Extraction and Separation of Total Polyphenols from Herba gei

Ultrasonic-assisted extraction followed by macroporous resin separation was adopted to prepare total polyphenols from Herba gei.Four crucial extraction parameters such as alcohol concentration,material/solvent ratio,extraction temperature were optimized to obtain the highest yield of total polyphenols by orthogonal array design.Under the optimized conditions of extraction at 60 ℃ for 60 min with 60% ethanol as the solvent and the material/solvent ratio of 1:10(g/ml),the yield of total polyphenols reached 1.77%.