Study on extraction and stability of pigment in Limonium Sinuatum
Bai Yu
Abstract
Bai Yu
Abstract
The stability and extracting conditions of the pigment in violet flowers of Forget-me-not(Limonium Sinuatum) was studied. This pigment is soluble in acid water, alcohol and methanol. The pigment is bluish- green in neuter and yellow under alkaline conditions. The maximum absorptive peak of ultraviolet-visible spectrogram of the pigment is at 530 ~540 nm. The best extracting solvent is 55% alcohol (pH 2). Paper chromatogram results show two spots. It is believed that the pigment is anthocyanins. The yield of the pigment is 15.5%. E 11c%m (540 nm)=36.55. The pigment is stable in acid and below 60 ℃ and under light conditions. The pigment can be destructed by H2O2 and Na2SO3, while Na2S2O3 and Vc have no effect on it. Fe3+ and Mg2+ undermine the stability of the pigment, while Na+, K+, Ba2+ and Ca2+ have no effect on it. Sucrose, starch, benzoic acid and acetic acid have no effect on the stability of the pigment.
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The stability and extracting conditions of the pigment in violet flowers of Forget-me-not(Limonium Sinuatum) was studied. This pigment is soluble in acid water, alcohol and methanol. The pigment is bluish- green in neuter and yellow under alkaline conditions. The maximum absorptive peak of ultraviolet-visible spectrogram of the pigment is at 530 ~540 nm. The best extracting solvent is 55% alcohol (pH 2). Paper chromatogram results show two spots. It is believed that the pigment is anthocyanins. The yield of the pigment is 15.5%. E 11c%m (540 nm)=36.55. The pigment is stable in acid and below 60 ℃ and under light conditions. The pigment can be destructed by H2O2 and Na2SO3, while Na2S2O3 and Vc have no effect on it. Fe3+ and Mg2+ undermine the stability of the pigment, while Na+, K+, Ba2+ and Ca2+ have no effect on it. Sucrose, starch, benzoic acid and acetic acid have no effect on the stability of the pigment.
Key concepts: Pigment, Chemistry, Acetic acid, Starch, Solvent, Extraction (chemistry), Methanol, Nuclear chemistry