Study on the effect of serum containing FuYuanHuoXue Recipe on the function of rat's osteoblasts in vitro

Objective:To study the effect of serum containing FuYuanHuoXue Recipe on the function of rat's osteoblasts in vitro and the mechanism of action.Methods:12 SD maleness rats aged 2 months were divided into two groups with 6 rats in each group at random,rats in one group(experimental group) undergone intragastric administration of FuYuanHuoXue decoction(20 g·kg-1) while equivalent normal saline was administrated in another group(control group) twice everyday,blood was obtained from abdominal aorta 14 days later.Osteoblasts were obtained from 2 neonatal rats and cultured respectively with fluid containing fetal bovine serum,serum from the rats in experimental group and control group.Function of osteoblasts was evaluated according to the measurement of proliferation,differentiation,mineralization and quantity of alkaline phosphatase(AKP).Results:①Proliferation of osteoblast:time effect:F=381.260,P=0.000;effect between groups:F=1 071.170,P=0.000;reciprocal effect between time and group:F=22.500,P=0.000.②Differentiation of osteoblast:time effect:F=159.950,P=0.000;effect between groups:F=62.120,P=0.000;reciprocal effect between time and group:F=2.400,P=0.029.③Mineralization of osteoblast:time effect:F=115.500,P=0.000;effect between groups:F=4.370,P=0.082;reciprocal effect between time and group:F=56.300,P=0.000.④content of AKP:time effect:F=2 175.670,P=0.000;effect between groups:F=1 201.370,P=0.000;reciprocal effect between time and group:F=428.870,P=0.000.Conclusion:FuYuanHuoXue decoction can incite proliferation and differentiation of rat's osteoblast in vitro owing to the promotion in secretion of alkaline phosphatase.