Effect of endothelin-1 on apoptosis and cell cycle of endothelial progenitor cells from rat bone marrow

AIM:To study the effects of endothelin-1(ET-1) on apoptosis and cell cycle of endothelial progenitor cells(EPC) from rat bone marrow. METHODS:Mononuclear cells were collected from rat bone marrow by density gradient centrifugation,cultured with Medium 199,and were identified by Di Ⅰ-acetylated low-density lipoprotein(Di Ⅰ-ac-LDL) and FITC-ulex europaeus agglutinin-Ⅰ(FITC-UEA-Ⅰ) double staining. EPCs collected at 5th day of culture were divided into 2 groups:Control group and ET-1 group (100 μmol/L),then were continuously incubated for different periods(24,48,72,96 h). The EPCs apoptosis and cell cycle were analyzed by flow cytometry(FCM). RESULTS:The EPCs apoptosis rate was higher in ET-1 group than in control group at 24,48,72,96 h(P0.01). Compared with 48 h ET-1 group,the EPCs apoptosis rate decreased in 72 h and 96 h ET-1 groups (P0.05,P0.01). The amount of EPCs in 24 h,48 h,72 h,96 h ET-1 groups in G0/G1 phase were significantly lower than those of control group(P0. 01). Compared with the 24 h,48 h,72 h,96 h control group,the amount of EPCs in 24 h,48 h,72 h,96 h ET-1 groups in S phase and in G2/M phase were significantly increased(P0.01). CONCLUSION:ET-1 can significantly stimulate both the proliferation and apoptosis of EPCs. With the prolongation of intervention duration,the impact on the proliferation becomes more prominent.