Effect of TLR4 and p-Akt on asthmatic airway smooth muscle migration and the impact of Xiaoqinglong decoction on the migration

Aim To explore the effect mechanism of TLR4 and p-Akt on the airway smooth muscle migration and the impact of Xiaoqinglong decoction on the migration in asthmatic rats.Methods 40 rats were divided into five groups including a control group(group A),4 weeks of asthmatic model group(group B),8 weeks of asthmatic model group(group C),4 weeks of Xiaoqinglong decoction group(group D) and 8 weeks of Xiaoqinglong decoction group(group E),with 8 rats in each group.The chronic asthmatic model was established by repeated inhalation of ovalbumin.The airway resistance was detected;the airway eosinophilic inflammation of airway wall was observed;the airway wall area,the bronchial smooth muscle area and the distance of smooth muscle to basement membrane of subcutaneous were measured by image analysis system;the expression of TLR4 and p-Akt protein was determined by immunohistochemical staining.Results The WAt /Pi and WAm/Pi in B group,C group,D group and E group were thicker than those in A group(P0.01),while the distance/Pi was shorter(P0.01).The WAt /Pi in B group and C group was thicker than that in E group,while the distance/Pi was shorter(P0.05);the content of TLR4 in airway smooth muscle of B group and C group was more than that of A group(P0.01) while C group was more than B group(P0.05).The content of TLR4 in airway smooth muscle of E group was less than that of C group and D group(P0.01).The content of p-Akt in airway smooth muscle of B group,C group and D group was more than that of A group(P0.01) while C group was more than B group(P0.05).The content of p-Akt in airway smooth muscle of E group was less than that of B group and C group and less than that of D group(P0.05).Conclusion TLR4 and p-Akt participate in the migration of asthmatic airway smooth muscle and through inhibiting TLR4 and p-Akt expression,Xiaoqinglong decoction may play a role in the airway remodeling of asthma.