Relationship between P53/VEGF Expression and Gastric Carcinoma

Purpose: In Order to investigate the relation between P53/VEGF (Vascular endothelial growth factor) expressions and the biological behavioer in gastric carcinoma. Methods: Human Tissue Specimens and Patien Information: 65 cases with gastric carcinoma were admitted to the Hospital Affiliated of Taian Medical College from Jan. 2004 to Jan. 2006, including 39 males and 26 females. They were aged 36–87 (58.67 ± 9.10). Neither radiotherapy nor chemotherapy were taken in preoperation period. Gastric carcinoma tissue specimens were obtained from normal gastric wall tissues in the same operation. Cancer specimens were classified based on the IUCC criteria in the stage of TNM, including well-differentiated adenoma 28 cases, poorly-differentiated 37 cases. Immunohistochemistry: All the light microscope specimens were fixed with formalin, embedded with paraffin, and sectioned at 5μm. Adopting immunohistochemistry S-P staining method. By the statistical analysis, Scores for percentage of positive cells were assingned as follows: >10% of cells positive. Weidner Analysis: Vessel count based on the criteria of Weidner. Appearing brown endothelial cells group or endothial cells group account for one vessel count. Count method is that each staining section choice three “plots” (40 magnification)in tumor interstitium areas. The statistial analysis of P53/VEGF adopted Chi square test, using nonparametric model to statistics analysis date. Results: There was close correlation between P53 and VEGF expression and clinical pathology characteristics of gastric carcinoma. The expression of VEGF was close correlated to gastric carcinoma expanding, and differentiated degree of tumor cells. The poor-differenitiated group expression 64.25% was obvious higher than well-differentitad group. The positive rate of VEGF was 68.26% in P53 positive group of 46 cases with gastric carcinoma tissues. The positive rate of VEGF was 26.19% in P53 negative group of 17 cases with gastric carcinoma tissue. The former was significanclly higher than the latter (p < 0.01). The P53 and VEGF positive expression of gastric carcinoma and WVC was significanclly higher than negative, There was obvious significance (p < 0.01). Conclusions: In our study, according to investigation of P53 and VEGF in expression of gastric carcinoma tissue. P53 and VEGF expression in gastric carcinoma tissue was close correlated to gastric carcinoma biological behavior.