2015•Hainan Yixueyuan xuebaoRequires access
Effect of ADSCs on immune function of C57 mice with thymomas induced by irradiation and mechanism
Kong Zhi-hu
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Abstract
Objective:To investigate that the effect of adipose-derived stem cells(ADSCs)on immune function of C57 mice with thymomas induced by irradiation,and plausible mechanism.Methods:A total of 32 cleaning degree C57BL/6mice were divided into four groups:control group of 8mice with non-irradiation;irradiation group of 8mice with irradiation;irradiation+ADSCs group of 8mice,thymoma model mice were injected 0.5mL ADSCs via tail vein at one day after last irradiation;non-irradiation+ADSCs group of 8 mice with the same ADSCs injection as irradiation+ADSCs group.Mice in four groups were sacrificed at 7th day after last irradiation,thymus index was measured by weight and thymus lymphocyte transformation rate was detected by MTY.Thymus lymphocyte cycle and T lymphocyte subsets were determined by flow cytometry.Results:Compared with irradiation group mice,lymphocyte transformation rate and thymus index was significantly higher in irradiation+ADSCs group mice.When detected by flow cytometry,CD4+T cell subsets and CD4+/CD8+from irradiation+ADSCs group mice increased,CD8+T cell subsets from irradiation+ADSCs group mice was decreased,there were statistical significance(P0.05).Conclusion:ADSCs may regulate the cell levels of mice thymus and protect immune function of mice.