Expression and Activity Deter mination of Functional Porcine Interferon-α Gene

[Objective]To clone Porcine Interferon-α(IFN-α)gene and determine its activity in yeast.[Methods]Interferon were induced and produced using phytohemagglutinin(PHA)in porcine peripheral blood lymphocytes.IFN-α gene was amplified by RT-PCR,and cloned into pPIC9K,the recombinant expression vetors were transformed into host strain Pichia pastoris GS115 by eletrization.After induced with methanol,The expression of IFN-α gene.was detected by SDS-PAGE and Western-blot.[Result]The porcine IFN-α gene were produced by RT-PCR and expressed in Pichia pastoris GS115 induced by methanol and had antigen specificity with IFN-α antibody.The molecular weight of this protein was 19 kD.[Conclusion]The functional porcine IFN-α gene were expressed in Pichia pastrois GS115.This study has paved the way for further exploring the IFN-α biological activity.