THE ROLE OF iNOS IN EGCG PROMOTING APOPTOSIS OF A549 CELLS

Objective To investigate the effect of inducible NOS(iNOS)on EGCG promoting apoptosis of A549cells.Methods A549cells were cultured in vitro and randomized to two groups:control group and model group.Optimal concentration of EGCG was obtained by MTT assay.The apoptosis effect of EGCG on A549cells was detected by flow cytometry.The expressions of iNOS and mRNA were detected by immunocytochemical western blotting and real time-PCR. Results The results showed that the differences of cell proliferation activity between 200mg/L EGCG group and other groups were significant(F=1 000.35,q=3.43-60.00,P0.05).The differences of the apoptosis rate of advanced stage A549cells between EGCG group and the control were significant(χ2=65.23,P0.05).Compared with the control group,EGCG could also reduce the expressions of iNOS protein and mRNA in A549cells(t=9.4,5.7;P0.05). Conclusion The apoptosis activity of A549cells treated with EGCG is likely to be related with downregulation of the expressions of iNOS protein and mRNA.